Wout Weghorst ended Wales' World Cup party and shattered their 19-match unbeaten home record in dramatic fashion as the Netherlands won 2-1 in Wednesday's Nations League clash in Cardiff.
Wales will head to Qatar in November for their first World Cup appearance in 64 years after beating Ukraine 1-0 in the play-off final on Sunday.
But Robert Page's side, who face USA, Iran and England in the group stage in Qatar, came back down to earth with a bump as they suffered a second successive Nations League Group A4 defeat after last week's loss in Poland.
In a thrilling finale, Rhys Norrington-Davies equalised for Wales in stoppage-time following Teun Koopmeiners' goal early in the second half.
Burnley striker Weghorst sealed the points for the Netherlands with just seconds left as the visitors ruined the longest unbeaten home run in Wales' history. For the first time since November 2018, Wales were beaten in front of their own fans.