The Champions league draw produced some familiar pairings on Monday with defending champion Barcelona facing Arsenal and Paris Saint-Germain again taking on Chelsea.
Barcelona eliminated Arsenal in the 2010 quarterfinals and in the round of 16 in 2011, and beat the English club in the 2006 final.
Arsenal will host the five-time champions on February 23 after advancing to the knockout round for the 16th straight season.
The Gunners will be hoping to end a five-year losing streak at the round-of-16 stage.
French champion PSG and Chelsea will be meeting for the third consecutive season in the knockout phase. Chelsea won in the quarterfinals in 2014 but PSG advanced from the round of 16 last season.
PSG, which set a competition record conceding only one goal in its group, could be the favorite against Chelsea for the first time.
"This is the third year we play against Chelsea so it has become some kind of a 'clasico' for us," PSG president Nasser Al-Khelaifi said.
"We will be ready."
Also, five-time champion Bayern Munich will play away first against 2015 runner-up Juventus, while record 10-time champion Real Madrid will travel to Roma.
Bayern beat the Italian champion Juventus in the quarterfinals when it last won the trophy three seasons ago.
"We had hoped for a little more luck in the draw, but I'm confident that we can turn in two great performances against Bayern. We've had an excellent run in Europe so far this season," Juventus vice president Pavel Nedved told the club's website.
"We were drawn in a tough group and did very well to come through it without too many difficulties. This means we'll also keep fighting in the last 16."
Juventus finished second to Manchester City in the Group D that included Germany's Borussia Monchengladbach and Spain's Sevilla.
"Nothing is impossible and you never know in football. We'll approach the game with humility and desire, fully aware that we're a strong team and have what it takes to compete for a place in the next round."
Still, there was one fresh look in the eight pairings as the two newcomers to the knockout rounds were drawn together: Gent will host Wolfsburg first in Belgium.
"It appears doable, without taking the rival lightly," Wolfsburg managing director Klaus Allofs said.
Meanwhile, Atletico Madrid CEO Clemente Villaverde said that PSV Eindhoven, who will face his side in the European Champions league round-of-16, are a "strong side".
"We're really happy to be here for the third year in a row. PSV are a strong side, they're a side with a lot of history in Europe and I'm sure they'll make life difficult for us," Efe quoted Villaverde as saying after the draw in Nyon, Switzerland.
In the other fixtures Dynamo Kiev will host Manchester City and Benfica will welcome Zenit St. Petersburg.
City's first leg in Kiev will be played in an empty stadium because Dynamo has been sanctioned by UEFA for racist incidents involving fans.
Manchester City director Txiki Begiristain warned that the English giants' round-of-16 tie with Dynamo Kiev is far from easy.
The 51-year old also revealed his delight that they have topped a UCl group for the first time. "It's never easy — they've shown that they have a good side by beating Porto and drawing with Chelsea," Begiristain told the club's website mcfc.com.
"It's going to be difficult for us but the most important thing is that we show we're improving in this competition and we'll fight to get to the quarters.
"The weather sometimes doesn't help but we have to be ready. At least we play in England — it's not the same but it's close. We're ready to try and improve in this competition and grow."