Valencia confirmed on Sunday five of the club's players and staff have tested positive for coronavirus, including Argentinian defender Ezequiel Garay.
Valencia said all those affected were "now at home, in good health and obeying self-isolation measures."
Garay, who was set to miss the rest of the season with a knee injury, became the first player in La Liga to confirm a positive test earlier on Sunday.
"Clearly I've got off on the wrong foot in 2020," Garay wrote on social media. "I've tested positive for the coronavirus, I'm fine and now just listening to the health authorities, at the moment in isolation."
Valencia confirmed more cases shortly after Garay's announcement. "Valencia CF confirm five positive cases of the COVID-19 coronavirus have been detected amongst first team staff and players.
“All of these persons are now at home, in good health and obeying self-isolation measures.”
Valencia played behind closed doors on Tuesday in the Champions League against Atalanta, the Serie A team based in Lombardy, one of Italy's worst-hit regions by the virus.
The club announced on February 28 they would be suspending all non-sporting activity with the first-team after a journalist attending the opening game against Atalanta was confirmed to have tested positive.
All engagement with the media before and after subsequent matches against Real Betis on February 29 and Alaves on Friday was cancelled, while a tribute to the club's former player David Villa was also called off.