Barcelona is joining a strike to protest against the Spanish government's actions in Catalonia's independence vote.
Also, outspoken defender Gerard Pique was jeered by spectators at Spain's training camp in Madrid on Monday.
Barcelona said none of its professional or youth teams will practice on Tuesday, and the club headquarters will be closed as a reaction to government attempts to stop the referendum on Sunday. Officials said more than 890 civilians and some 430 members of the police force were injured.
Girona also suspended practice on Tuesday, and Espanyol, the other Catalan club in the Spanish league, will have its players undergo physical activities behind closed doors at its training camp.
Pique, who supported the referendum, was booed constantly as Spain practiced. He endured chants, sometimes laced with expletives, of "Spain is your nation." Some held cards against him, including one that read "Out Pique." Policed deemed one of the cards too offensive and removed it. Pique is often jeered by fans when Spain plays outside of Catalonia.
He was in tears on Sunday after the Spanish league game against las Palmas as he talked about confrontations between Catalan voters and police, who used batons and fired rubber bullets to try and stop voters across the northeastern region. He criticized police again on Monday on Twitter.
Pique said after on Sunday that if the national team considered him a nuisance, he would have no problem stepping aside.
Spain is preparing for World Cup qualifiers, the next on Friday against Albania in the southeastern city of Alicante.
Barcelona tried to postpone its home match on Sunday during the referendum but the league denied its request, so it closed Camp Nou to spectators to send a protest message and avoid losing points and being sanctioned. It beat las Palmas 3-0.
Barcelona president Josep Bartomeu said the decision to play behind closed doors was one of the hardest he had to make at the club. He consulted club officials, coaches and players.
"We knew that an empty Camp Nou would send a powerful message," he said in a news conference on Monday. "Everybody would be asking about what was happening in Catalonia. The game was broadcast around the world. It was an extraordinary measure for an extraordinary moment."