Spain's Alexia Putellas, the reigning Ballon d'Or winner, has been ruled out of Euro 2022 after tearing the anterior cruciate ligament in her left knee on the eve of the tournament.
"Tests carried out on the Spain international this afternoon ... have confirmed the national team captain suffered a ruptured anterior cruciate ligament," the Spanish football federation said.
Putellas, 28, was expected to be one of the stars of the tournament that begins in England on Wednesday.
Spain had already lost their all-time top goalscorer Jennifer Hermoso to injury.
Hermoso, who recently signed for Mexican club Pachuca from Barcelona, sustained a knee injury last month shortly after joining up with the Spain squad.
Spain were considered one of the favourites as they bid for a first major women's tournament success.
Spain open their campaign against Finland on July 8 in Milton Keynes.